Data Gridview: how do i stop: values of Data Grid view Automatically Erises Itself

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C#

GUI

I am Using Data Grid view In My Windows Project:
After Enter Data in Data Grid view In my form I have to chose Radio Button Option:
Then at Button click Should Submit Data s in to Data base:

While i am chose Radio Button Option Sometimes All values of Grid view Automatically Erises Itself.All Data Grid view Rows of Automatically gone;

How do i stop this
How can i solve this problem Please Help me