Read RFID Tag information using ASP.Net, C#

Since the default security level of a web browser restricts any code running inside it from accessing the machines resources, file system, registry, ..., no it wouldn't. It would, but when you deploy your component to the client you have to alter the browsers security settings to tell the browser to trust the source of the component. Truthfully, I wouldn't be writing a web app to handle a hardware device. I'd be writting either a Windows Forms app for the client side RFID scanning interface having it send the data to the database on the web server via a Web Service running on that. But, then again, I have no what you're doing with the RFID scanner and what your application does.