ASP.net Error Access is denied when Accessing file from IIS virtual Directory

Thanks in advance,
 
I have made a virtual Directory in IIS and has given permissions to IIS_IUSRS (Full Control) and Even added everyone,But when i am accessing attached file from the webpage its giving error
 

Quote:

Exception Details: System.ComponentModel.Win32Exception: Access is denied

 
while from Visual studio when i click on the link , files are opening , Only when i browse from IIS it gives error.when i adds identity impersonate ="true"
in web.config,it gives error on page load
 

Quote:

An ASP.NET setting has been detected that does not apply in Integrated managed pipeline mode.

 
Any suggestions ?