As you not mentioned which language you use i try it with C#,when you press a button the calculation is done and show the result in 4th textbox..
protected void Button1_Click(object sender, EventArgs e)
{
double value1, value2, value3, value4;
value1 = Convert.ToDouble(TextBox2.Text);
value2 = Convert.ToDouble(TextBox3.Text);
value3 = Convert.ToDouble(TextBox4.Text);
value4=value1-(value2+value3);
TextBox5.Text = value4.ToString();
}