Exactly how you do this will depend on what your data format is like. If it is always "one character, followed by four numerics, then it is trivial:
string inp = "A0001";
string alpha = inp.Substring(0,1);
string numeric = inp.Substring(1);
If on the other hand it is a variable length non-numeric at the front, then you would be better off using a Regex:
string inp = "A0001";
Regex regex = new Regex(@"(?<Alpha>[^\d]*)(?<Numeric>\d+)");
Match m = regex.Match(inp);
string alpha = m.Groups["Alpha"].Value;
string numeric = m.Groups["Numeric"].Value;