Of course you can!
<asp:sqldatasource id="YourSqlDataSource" runat="server" xmlns:asp="#unknown">
ConnectionString="<%$ ConnectionStrings:YourConnectionString %>"
UpdateCommand="UPDATE YourTable SET YourColumn = '0';
UPDATE Your2ndTable SET Your2ndColumn = '1' WHERE ID = @ID;">
<updateparameters>
<asp:parameter name="ID" />
</updateparameters>
</asp:sqldatasource>