If your sound file is a .wav file, you can try this:
protected void button1_Click(object sender, EventArgs e)
byte result = System.IO.File.ReadAllBytes("sound.wav");
System.IO.MemoryStream ms = new System.IO.MemoryStream(result);
ms.Position = 0;
System.Media.SoundPlayer soundPlayer = new System.Media.SoundPlayer(ms);
If your audio file isn't a .wav file, then have a look here:http://www.crowsprogramming.com/archives/58
You can also convert your audio file to a .wav file.
Have a look at this list of audio conversion software
Hope this helps.