use
ValidationGroup
Property
<asp:requiredfieldvalidator id="Req9" runat="server" ValidationGroup="a"
ErrorMessage="Enter PINno"></asp:requiredfieldvalidator>
<asp:requiredfieldvalidator id="Req10" runat="server" controltovalidate="TextBox2" ValidationGroup="b"
ErrorMessage="Enter PINno"></asp:requiredfieldvalidator>
<asp:button id="btnsubmit" runat="server" ValidationGroup="a"
Text="Button1" onclick="btnsubmit_Click1"/>
<asp:button id="btnsubmit1" runat="server" ValidationGroup="b"
Text="Button2" onclick="btnsubmit1_Click1" />
Group the Textbox1 with Button1 and Textbox2 with Button2