You cannot remove/modify any open file. You cannot do it with any executable file which is loaded by any currently running project.
You need to find out who is holding your file. Sometimes it could be Visual Studio host; I also knew the cases when a Visual Studio help agent does it. Close all versions of Visual Studio, run "Task Manager" (Ctrl+Alt+Delete) to kill unwanted processes. Also, you could simply run some of your applications which you might not correctly terminate.
If you cannot manually find it out, use Sysinternals Process Manager from Sysinternals Suite, see
http://technet.microsoft.com/en-us/sysinternals/bb842062[
^] or the application WhoLockMe (please find by yourself, I don't have a link, sorry).
—SA