To work with a section of an image, you create a new Bitmap object, get it's Graphics context, and draw into it from the original image. This draws a 30x30 pixel section of an image from the point (100,100) in the original image:
System.Drawing.Bitmap bmp = new Bitmap(30, 30);
Image myPic=System.Drawing.Image.FromFile(@"F:\Temp\MyPic.jpg");
using (Graphics g = bpm.CreateGraphics())
{
g.DrawImage(myPic, new Rectangle(0,0, 30, 30), new Rectangle(100,100, 30, 30), GraphicsUnit.Pixel);
}