i think you have used Ajax toolkit/script manager in your code. you need to add some tags in web.config.
Install ajax extesion and add following tag in web.config
Within the
<httphandlers> </httphandlers>
section, add the following entry
<add verb="GET" path="ScriptResource.axd" type="Microsoft.Web.Handlers.ScriptResourceHandler" validate="false" />
check the following link
http://geekswithblogs.net/ranganh/archive/2007/07/15/113963.aspx