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Based on the code, it looks like a dropdownlist does exists from before. In the RowDataBound event you are trying to populate data in that pre-existing dropdown. Since, it pre-exists, you can define a selected change index for it.

In case you want to define it at runtime in RowDataBound, then you need to add this:

AddHandler ddlFilter.SelectedIndexChanged, AddressOf Me.ddl2_SelectedIndexChanged
ddlFilter.AutoPostBack = true

    
Private Sub ddlFilter_SelectedIndexChanged(ByVal sender As Object, ByVal e As EventArgs)
        'your code 
End Sub