Hi Swaroopa check the following code works for you.Add the items to Listbox out of for loop.
string get_value = "";
for (int i = 0; i < gvqueriescheck.Rows.Count; i++)
{
CheckBox chbTemp = GridView1.Rows[i].FindControl("chkcheckquery") as CheckBox;
if (chbTemp.Checked)
{
get_value = gvqueriescheck.Rows[i].Cells[2].Text;
}
}
ListBox1.Items.Add(get_value);