So set up two button Click event handlers, and a Form level private DateTime variable.
On one timer, set the variable to the current date and time, and on the other, subtract it from the current date and time. You then have a TimeSpan you can display:
private DateTime startTime = DateTime.Now;
void butStart_Click(Object sender, EventArgs e)
{
startTime = DateTime.Now;
}
void butShow_Click(Object sender, EventArgs e)
{
TimeSpan diff = DateTime.Now - startTime;
labDifference.Text = diff.ToString();
}