how to set value of Wpf Datagrid cell in code behind

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WPF

DataGrid

Hi,Can anybody help me i need set value of paricular celll
im not getting how to bind..i used following method..
it creates a empty cell my requirment is populate it with some value

column = new DataGridTextColumn();
                    column.Header = Header[i];
                    column.Binding = new Binding(dgCols[i]);
                    dgCourseInfo.Columns.Add(column);
                    if (i + 1 == Header.Count)
                    {
                        column = new DataGridTextColumn();
                        column.Header = Header[i];
                        column.Binding = new Binding();
                        dgCourseInfo.Columns.Add(column);

                    }