how to Barcode printing rows and columns on A4 paper with vb.net?

Usually the code 3 of 9 family needs no separation character.

If your system uses True Type fonts, here's a good 3 of 9 (39) font: http://download.cnet.com/Free-TrueType-Code-39-Barcode-Font/3000-2190_4-10067143.html[^]

Most 3 of 9 fonts require start and end characters, usually *. So if you want the barcode to read 1234, you should have a string *1234* and change the entire string to the 39 font.