In Visual Studio, Right click on class file and go to properties.
Check for BuildAction property of file. This should be set to "Compile"
then you can create instance as below
WebApplication1.App_Code.BAL.BusinessAccessLayer_Class d = new WebApplication1.App_Code.BAL.BusinessAccessLayer_Class();
if you set using directive like below you can create instance without full class name.
using WebApplication1.App_Code.BAL;
namespace WebApplication1
{
public partial class _Default : System.Web.UI.Page
{
protected void Page_Load(object sender, EventArgs e)
{
BusinessAccessLayer_Class d = new BusinessAccessLayer_Class();