You are missing the '' in if condition,
Try like this,
<script language="javascript" type="text/javascript">
function Validate()
{
if (document.getElementById('<%=Textbox1.ClientID %>').value == "")
{
alert("Please Enter Your Field!");
document.getElementById('<%=Textbox1.ClientID %>').focus();
return false;
}
}
</script>
And call the Validate function in ur button,
<asp:Button Id="Button1" runat="server" OnClientClick="return Validate();" Text="Button" />