You basically need to "load the ISO" first to access the files contained inside.
Have a look at the following:
.NET DiscUtils[
^]
It will allow you to do the following:
using (FileStream isoStream = File.Open(@"C:\temp\sample.iso"))
{
CDReader cd = new CDReader(isoStream, true);
Stream fileStream = cd.OpenFile(@"Folder\Hello.txt", FileMode.Open);
}