Why don't you give the image url from you source page if you want that on the pageload ?
This should do the job-
<asp:image id="Image1" runat="server" imagealign="Middle" imageurl="~/Images/LiveRadio.jpg" xmlns:asp="#unknown"></asp:image>
Make sure that you have browsed the url instead of copy past or writing manually.
Hope, this helps :)
Update
Make sure that you have commented following
protected void Page_Load(object sender, EventArgs e)
{
}