You only need one ScriptManager tag. Do not place it so low in your page. The best position for it is right after the opening for tag in your Master Page.
<form id="form1" runat="server">
<asp:ScriptManager ID="ScriptManager1" runat="server" EnablePageMethods="true" EnableScriptGlobalization="true"
EnableScriptLocalization="true">
</asp:ScriptManager>
</form>