Because you are not drawing on the screen, you are drawing on a bitmap:
obj_Graphics = Graphics.FromImage(obj_Bitmap);
If you want to draw it i=on teh screen, then handle the Paint event for your form, or a panel on your form and use the Graphics object you are handled in the EventArgs parameter:
e.Graphics.DrawLine(new Pen(new SolidBrush(Color.Black), 5), 100, 500, 400, 500);