trace.axd is not working

See more:

IIS7

C#

ASP.NET

IIS

C#4.0

tracing

localhost/Prafulla/homepage.aspx/trace.axd is not working why?
I did the changes in Web.config file also.

<system.web>
    <trace enabled="true" localOnly="false" pageOutput="true" requestLimit="40"/>
  </system.web>

when I am running my application locally it works but when I am running my application in from IIS it is not working.

localhost:3657/Prafulla/homepage.aspx/trace.axd(WORKING)
localhost/Prafulla/homepage.aspx/trace.axd(NOT WORKING)

Suggest me how would I overcome from this.
Thanks.