Web Service gives error after changing version of .Net framework from 3.5 to 4.0

I am creating an intranet application which needs the windows loginname. I am trying to retrieve the user id from System.Net.CredentialCache.DefaultNetworkCredentials and using this user Id to retrieve First and Last name of the user using the web service. It's worked fine in my localhost machine. But the problem is when I am trying to access the site from Development server it is showing the error:

The request failed with HTTP status 401: Access Denied

But the same code is working fine in Test environment.I don't have any idea why these both environment behaving differently. I am using windows Integrated authentication and Anonymous access disabled.

This application was in .net 3.5 and i changed to .net framework 4.0 version using VS2010. This web service is working perfectly in 3.5 version so my question is this version change will make any difference to web service?

Please help me to figure out this issue.

Dim consumeWebService As ws-ProfileService
consumeWebService = New ws-ProfileService
consumeWebService.PreAuthenticate = True
consumeWebService.Credentials = System.Net.CredentialCache.DefaultNetworkCredential

Exception:
 The request failed with HTTP status 401: Unauthorized.
Description: An unhandled exception occurred during the execution of the current web request. Please review the stack trace for more information about the error and where it originated in the code.

Exception Details: System.Net.WebException: The request failed with HTTP status 401: Unauthorized.

Source Error:

An unhandled exception was generated during the execution of the current web request. Information regarding the origin and location of the exception can be identified using the exception stack trace below.

Stack Trace:


[WebException: The request failed with HTTP status 401: Unauthorized.]
   System.Web.Services.Protocols.SoapHttpClientProtocol.ReadResponse(SoapClientMessage message, WebResponse response, Stream responseStream, Boolean asyncCall) +2298
   System.Web.Services.Protocols.SoapHttpClientProtocol.Invoke(String methodName, Object[] parameters) +345
   STOP.gov.cdc.activedirectory_ws.ProfileService.GetUserProfile(String UserID) in C:\Projects\STOP\STOP\Web References\gov.cdc.activedirectory-ws\Reference.vb:81
   STOP._Default.Page_Load(Object sender, EventArgs e) in C:\Projects\STOP\STOP\Default.aspx.vb:54

[WebException: Praseetha's findings]
   STOP._Default.Page_Load(Object sender, EventArgs e) in C:\Projects\STOP\STOP\Default.aspx.vb:107
   System.Web.UI.Control.LoadRecursive() +116
   System.Web.UI.Page.ProcessRequestMain(Boolean includeStagesBeforeAsyncPoint, Boolean includeStagesAfterAsyncPoint) +2809

Thanks in advance

Tags: Visual Basic, ASP.NET