Hi,
Use the below mentioned code.It will check whether the selected file has been uploaded or not .If not uploaded it won't open the dialog for second file upload.
How to check file exist or not in Ajax toolkit file uploadcontrol[
^]
Before doing that add a onclickevent to your second file upload control say FU2.
whick will be like
<asp:ajaxfileupload runat="server" id="FU2" onclick="return checkAttachments()" xmlns:asp="#unknown">
Regards
Hsakarp.