Add JS file programaticaly and try
Dim ObjLocJsForJquery = New HtmlGenericControl()
ObjLocJsForJquery.TagName = "script"
ObjLocJsForJquery.Attributes.Add("type", "text/javascript")
ObjLocJsForJquery.Attributes.Add("language", "javascript")
ObjLocJsForJquery.Attributes.Add("src", Page.ResolveClientUrl("~/Js/jquery-1.8.2.min.js"))
Page.Header.Controls.Add(ObjLocJsForJquery)
And Check.