2.dynamically button not firing while the button click

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ASP.NET

SQL-Server

Dynamically button not firing while the button click from panel.

What I have tried:

btn_Reply.ID = "btn_ReplyUser" + i;
btn_Reply.Text = "Reply";
btn_Reply.Width = 250;
btn_Reply.AutoPostBack = true;
btn_Reply.Command += new CommandEventHandler(btn_Reply_command);
btn_Reply.Click += new System.EventHandler(btn_ReplyUser);
btn_Reply.CausesValidation=false;
this.form1.Controls.Add(btn_Reply)
pnlpopup.Controls.Add(btn_Reply)

private void btn_ReplyUser(object sender, EventArgs e)
{
//throw new NotImplementedException();
TextBox txt_re = new TextBox();
txt_re.Text = "Hello";
}