Error in file upload from client to server.

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ASP.NET

we are try to upload some file from file upload control from client to server. this code is proper working in local machine. and upload files. but after hosting it gives following error:

Error: Access to the path G:PleskVhostsexamcart.inhttpdocsDochindi.jpg is denied.

we are using asp.net 4.0. if there is any permission problem. then how we provide permission on remote server or iis.

What I have tried:

if (FileUpload1.HasFile)
{
    FileUpload1.SaveAs(Server.MapPath("~/Doc/") + TxtFileName.Text.Trim() + 
    System.IO.Path.GetExtension(FileUpload1.PostedFile.FileName));
}

we also tried following. but generate same error:

if (FileUpload1.HasFile)
{
    FileUpload1.SaveAs(Server.MapPath("~//Doc//") + TxtFileName.Text.Trim() + 
    System.IO.Path.GetExtension(FileUpload1.PostedFile.FileName));
}