Add a class to the header and the item template of the column you want to resize, then simply adjust the width of items with that class. Below I've added the cladd "pdCol" to the personalDetail column and added a button that resizes those columns when clicked
<asp:GridView ID="GridPartDet" runat="server" AutoGenerateColumns="False" CellPadding="3"
CssClass="ph clr width100per" OnRowCommand="GridPartDet_RowCommand" TabIndex="24">
<Columns>
<asp:TemplateField HeaderText="S No">
<ItemTemplate>
<%# ((GridViewRow)Container).RowIndex + 1%>
</ItemTemplate>
<ItemStyle Width="8%" />
</asp:TemplateField>
<asp:TemplateField HeaderText="Action">
<ItemTemplate>
<asp:LinkButton ID="LinkBtnEdit" runat="server" CausesValidation="false" CommandName="EditRow"
CssClass="gridCommand" TabIndex="25" Text="Edit"></asp:LinkButton>
<asp:LinkButton ID="LinkBtnDelete" runat="server" CausesValidation="false" CommandName="DeleteRow"
CssClass="gridCommand" TabIndex="26" Text="Delete"></asp:LinkButton>
</ItemTemplate>
<ItemStyle Width="10%" />
</asp:TemplateField>
<asp:TemplateField HeaderText="Personal" Visible="false">
<ItemTemplate>
<asp:Label ID="LbPersonal" runat="server" Text='<%# Eval("Personal") %>'></asp:Label>
</ItemTemplate>
<ItemStyle Width="20%" />
</asp:TemplateField>
<asp:TemplateField HeaderText="PersonalDetail">
<ItemTemplate>
<asp:Label ID="LblPartID" runat="server" Text='<%# Eval("PersonalDetail") %>'></asp:Label>
</ItemTemplate>
<ItemStyle Width="10%" CssClass="pdCol" />
<HeaderStyle CssClass="pdCol" />
</asp:TemplateField>
<asp:TemplateField HeaderText="PartRemark">
<ItemTemplate>
<asp:Label ID="LblPartDetails" runat="server" Text='<%# Eval("PartRemark") %>'></asp:Label>
</ItemTemplate>
<ItemStyle Width="40%" />
</asp:TemplateField>
</Columns>
<FooterStyle BackColor="#CCCCCC" />
<HeaderStyle BackColor="LightGray" Font-Bold="True" CssClass="ForeColor" />
</asp:GridView>
<button id="btnResize">Resize</button>
<script>
$(document).ready(function () {
$("#btnResize").click(function (e) {
e.preventDefault();
$(".pdCol").css("width", "500px");
});
});
</script>