Insert in gridview

To insert a new row of data @ runtime then follow Mohd's post.

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Populating the GridView with new data - use DataSource then DataBind.

To update or refresh your current datasource then just DataBind again.

GridView1.DataSource = "DataObject"
GridView1.DataBind()

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If you wanting to add a new GridView at runtime then the best way would be to have a PlaceHolder control available.

var gridView = new GridView();
gridView.DataSource = "DataObject";
gridView.DataBind();

Placeholder1.Controls.Add(gridView);