You can't get the content of an image tag, img or asp:Image. You can show the preview if you want, but you have to submit the file as part of a form submission and read it as a normal file.
Google "asp.net upload image file" and you'll find examples of how to do this.
<asp:FileUpload ID="myFile" runat="server" onchange="showpreview(this);" />
<img id="imgpreview" />
<asp:Button ID="btnSubmit" OnClick="btnSubmit_Click" runat="server" />
<script type="text/javascript">
function showpreview(input) {
if (input.files && input.files[0]) {
var reader = new FileReader();
reader.onload = function (e) {
$('#imgpreview').css('visibility', 'visible');
$('#imgpreview').attr('src', e.target.result);
}
reader.readAsDataURL(input.files[0]);
}
}
</script>