You have to Group your all Radio Button With the same Name like below,
<pre lang="xml"><asp:GridView ID="GridView1" runat="server" AutoGenerateColumns ="False" OnSelectedIndexChanged="GridView1_SelectedIndexChanged1">
<Columns>
<asp:BoundField DataField ="Column1" HeaderText ="ProductName" />
<asp:BoundField DataField ="Column2" HeaderText ="ProductID" />
<asp:TemplateField >
<ItemTemplate>
<asp:RadioButton ID ="radioButton1" runat="server" GroupName ="productDB" AutoPostBack ="true" OnCheckedChanged ="Rb1_CheckChanged" />
<asp:RadioButton ID ="radioButton2" runat="server" GroupName ="productDB" AutoPostBack ="true" OnCheckedChanged ="Rb2_CheckChanged" />
<asp:RadioButton ID ="radioButton3" runat="server" GroupName ="productDB" AutoPostBack ="true" OnCheckedChanged ="Rb3_CheckChanged" />
</ItemTemplate></asp:TemplateField>
</Columns>
</asp:GridView>
As you can see i have given a Same name to all 3 radio buttons {GroupName =productDB}
I hope this will help you.