IIS Sub-Directory is not accessible

See more:

VB

ASP.NET

IIS

MS-Excel

Hi Professionals,

I have developed a web application called Accounting Software using VB.Net...

In this program, I used to export some automated excel sheet in to the sub directory called "EXCEL"...

If I run the application through VS2005 [http://localhost:1440/Account/] its perfectly running...

After I published the web application, and paste in to IIS Directory
(c:\inetpub\wwwroot\Account\Excel), The sub directory "Excel" is not accessible...

I go through some permission issues also, that is

*) Right Click the folder -> Security Tab -> Checked FULL CONTROL and Done Advanced options too...

But its not working... Please help me... Its very urgent..