Hi,
I use javascript for validating controls.
You can my code for validating your control with some changes.
<html xmlns="http://www.w3.org/1999/xhtml">
<head runat="server">
<title></title>
<script language ="javascript" >
function f1() {
if (parseInt(document.getElementById("DropDownList1").selectedIndex) > 0) {
alert("you selected option");
}
else {
alert("you are Not selected ");
}
}
</script>
</head>
<body>
<form id="form1" runat="server">
<div>
<div id="divid" runat="server"></div>
<input type ="file" id="fileupload1" />
<input type ="button" value="change" onclick ="f1()" />
<asp:button id="Button1" runat="server" text="Button" onclick="Button1_Click" xmlns:asp="#unknown" />
<select name="DropDownList1" id="DropDownList1">
<option value="dfgdfgfdg">dfgdfgfg</option>
<option value="tytytrytry">uytuytuy</option>
<option value="ghj">hjghj</option>
<option value="ghjhhj">yuiyui</option>
</select>
</div>
</form>
</body>
</html>
You can change above to achieve your requirement.
All the Best