You need to go like this..
first of generate Row Data Bound event, which will tack care for your check-box/radio button which is checked automatically to previous entry while your bind the data to your grid.
Radio button checked issue for row data bound[
^]
bind-radio-button-list-to-column-in-gridview[
^]
for highlighting your radio button you can use css for it..
input[type=radio]:checked {
border: 1px solid black;
color: red;
}
it will highlight your radio button which is selected or checked.