try this one....
<asp:RegularExpressionValidator id="RegularExpressionValidator1"
ControlToValidate="TextBox1"
ValidationExpression="\d+"
Display="Static"
EnableClientScript="true"
ErrorMessage="Please enter numbers only"
runat="server"/>
or
function fncInputNumericValuesOnly()
{
if(!(event.keyCode==45||event.keyCode==46||event.keyCode==48||event.keyCode==49||event.keyCode==50||event.keyCode==51||event.keyCode==52||event.keyCode==53||event.keyCode==54||event.keyCode==55||event.keyCode==56||event.keyCode==57))
{
event.returnValue=false;
}
}