Hi
Try like this,
You cannot modify the Items , once it is databound.
option is , you can modify the data source as below.
List<string> lst = new List<string>();
lst.Add("one");
lst.Add("two");
lst.Add("three");
lst.Insert(0, "");
comboBox1.DataSource = lst;
DataTable dt = new DataTable ();
dt.Columns.Add("Numbers", typeof(string));
dt.Rows.Add("one");
dt.Rows.Add("two");
dt.Rows.Add("three");
DataRow newrow = dt.NewRow();
newrow["Numbers"] ="";
dt.Rows.InsertAt(newrow, 0);
comboBox1.DataSource = dt;
comboBox1.DisplayMember = "Numbers";</string></string>