It's Very Simple :
If System.IO.File.Exists("Path of the Folder with File Name") Then
MessageBox.Show("Exist")
Else
MessageBox.Show("Not Exist")
End If
Or
If My.Computer.FileSystem.FileExists("Path of the Folder with File Name") Then
MessageBox.Show("Exist")
Else
MessageBox.Show("Not Exist")
End If