when i use order by clause my first row in gridview will be disabled

First, this is not a question. Then, your SQL has nothing to do with the effect you describe, not directly. This report tells us very little. There are many ways to screw up things; and your information is not sufficient to determine which way is your favorite... :-)

Some code sample might help. Try to reproduce this problem is the code sample, as short as possible, but complete. If you can do it, use "Improve question" above.
--SA

This is not a well framed question! We cannot work out what you are trying to do/ask from the post. Please elaborate and be specific.
Use the "Improve question" link to edit your question and provide better information.