In the button click event, if the user authentication fails, set the text propert of your textbox and label to empty string.
Something like:
protected void Button1_Click(object sender, EventArgs e)
{
TextBox1.Text = "";
Label1.Text = "";
}
What is the label for?
You should also have a label which notifies the user about the authentication failure (I have added that as a comment in the code).