The easiest way is to hold the notes as individual WAV or MP3 files - for a Guitar tuner where you need a small number of notes that is fine - and play them on the keystroke.
If you save them as WAV files, you can add them to your application Resources, and play them very easily:
SoundPlayer keyOfC= new SoundPlayer(Properties.Resources.NoteC);
keyOfC.Play();
Beyond that, there is Console.Beep - but it may not work in all OS's - it allows you to specify the frequency and duration:
Console.Beep(261, 10000);
will play a C for ten seconds